mouse monoclonal anti-ha.11 antibody (clone 16b2) (Covance)
90
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Covance
mouse monoclonal anti-ha.11 antibody (clone 16b2)
Mouse Monoclonal Anti Ha.11 Antibody (Clone 16b2), supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha%2E11+antibody/anti+ha/pm40440169-174-2-12
Average 90 stars, based on 1 article reviews
Mouse Monoclonal Anti Ha.11 Antibody (Clone 16b2), supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha%2E11+antibody/anti+ha/pm40440169-174-2-12
Average 90 stars, based on 1 article reviews
mouse monoclonal anti-ha.11 antibody (clone 16b2) - by Bioz Stars,
2026-09
90/100 stars
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Blocking Assay:Article Title: Characterization of the HHV-6B U20 Immunoevasin. Article Snippet: Roseoloviruses (human herpesvirus 6A [HHV-6A], -6B, and -7) infect .90% of the human population during early childhood and are thought to remain latent or persistent throughout the life of the host.. As such, these viruses are among the most pervasive and stealthy of all viruses; they must necessarily excel at escaping immune detection throughout the life of the host, and yet, very little is known about how these viruses so successfully escape host defenses.. Here, we characterize the expression, trafficking, and posttranslational modifications of the HHV6B U20 gene product, which is encoded within a block of genes unique to the roseoloviruses. Article Title: Exploring the Expression and Function of cTyro3, a Candidate Zika Virus Receptor, in the Embryonic Chicken Brain and Inner Ear. Article Snippet: The primary antibodies were diluted in blocking solution as follows: anti-GFP (1:1000, rabbit IgG, Invitrogen, Waltham, MA, USA, A11122), HA.11 (1:500, Article Title: Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca 2+ homeostasis with adipose tissue lipolysis Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alpha-MSH Millipore Cat# AB5087; RRID: AB_91683 Alpha-Tubulin Sigma-Aldrich Cat# T6199; RRID: AB_477583 Alexa Fluor 594 chicken anti-mouse ThermoFisher Cat# A-21201; RRID: AB_2535787 Alexa Fluor 488 chicken anti-rabbit ThermoFisher Cat# A-21441; RRID: AB_2535859 Alexa Fluor 488 donkey anti-sheep ThermoFisher Cat# A-11015; RRID: AB_2534082 Alexa Fluor 594 donkey anti-rabbit ThermoFisher Cat# A-21207; RRID: AB_141637 Alexa Fluor 647 donkey anti-rabbit ThermoFisher Cat# A32795; RRID: AB_2762835 Anti-mouse HRP GE Healthcare UK Limited Cat# NA931; RRID: AB_772210 Anti-Rabbit HRP GE Healthcare UK Limited Cat# NA934; RRID: AB_2722659 b-Actin Sigma-Aldrich Cat# A2066; RRID: AB_476693 GFP Aves Labs Cat# GFP-1010; RRID: AB_2307313 HA.11 Article Title: A ubiquitin variant-based affinity approach selectively identifies substrates of the ubiquitin ligase E6AP in complex with HPV-11 E6 or HPV-16 E6 Article Snippet: Where indicated, HA-tagged proteins were detected by the Article Title: HP1 drives de novo 3D genome reorganization in early Drosophila embryos Article Snippet: HP1 Developmental Studies Hybridoma Bank C1A9 (validated by western blot and ChIP in HP1-KD in ED Fig. 1g and 3b) Article Title: Sumoylation of SAP130 regulates its interaction with FAF1 as well as its protein stability and transcriptional repressor function. Article Snippet: The following antibodies were purchased: Article Title: LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding Article Snippet: Cells were fixed in 10% formaldehyde, permeabilized in 0.1% Triton X-100, and immunostained with antibody against FLAG M2 (Sigma, 1:500), HA.11 (Covance, 1:500), TOM20 (Santa Cruz BioTech, 1:500) or HSP60 (Santa Cruz BioTech, 1:500). Article Title: Tandem Immunoaffinity Purification Using Anti-FLAG and Anti-HA Antibodies. Article Snippet: The immunoaffinity purification of target proteins followed by the identification and characterization of associated proteins by mass spectrometry is a widely used technique.. An immunoaffinity purification bears resemblance to a standard immunoprecipitation; however, the end product for mass spectrometric analysis in the femtomole (10) to attomole (10) range is required to be of exceptional purity.. This high degree of sensitivity in detection renders it of extreme importance to eliminate most if not all of the nonspecific background proteins and can be achieved by performing a tandem affinity purification (TAP). Marker:Article Title: Characterization of the HHV-6B U20 Immunoevasin. Article Snippet: Roseoloviruses (human herpesvirus 6A [HHV-6A], -6B, and -7) infect .90% of the human population during early childhood and are thought to remain latent or persistent throughout the life of the host.. As such, these viruses are among the most pervasive and stealthy of all viruses; they must necessarily excel at escaping immune detection throughout the life of the host, and yet, very little is known about how these viruses so successfully escape host defenses.. Here, we characterize the expression, trafficking, and posttranslational modifications of the HHV6B U20 gene product, which is encoded within a block of genes unique to the roseoloviruses. Article Title: Exploring the Expression and Function of cTyro3, a Candidate Zika Virus Receptor, in the Embryonic Chicken Brain and Inner Ear. Article Snippet: The primary antibodies were diluted in blocking solution as follows: anti-GFP (1:1000, rabbit IgG, Invitrogen, Waltham, MA, USA, A11122), HA.11 (1:500, Article Title: Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca 2+ homeostasis with adipose tissue lipolysis Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alpha-MSH Millipore Cat# AB5087; RRID: AB_91683 Alpha-Tubulin Sigma-Aldrich Cat# T6199; RRID: AB_477583 Alexa Fluor 594 chicken anti-mouse ThermoFisher Cat# A-21201; RRID: AB_2535787 Alexa Fluor 488 chicken anti-rabbit ThermoFisher Cat# A-21441; RRID: AB_2535859 Alexa Fluor 488 donkey anti-sheep ThermoFisher Cat# A-11015; RRID: AB_2534082 Alexa Fluor 594 donkey anti-rabbit ThermoFisher Cat# A-21207; RRID: AB_141637 Alexa Fluor 647 donkey anti-rabbit ThermoFisher Cat# A32795; RRID: AB_2762835 Anti-mouse HRP GE Healthcare UK Limited Cat# NA931; RRID: AB_772210 Anti-Rabbit HRP GE Healthcare UK Limited Cat# NA934; RRID: AB_2722659 b-Actin Sigma-Aldrich Cat# A2066; RRID: AB_476693 GFP Aves Labs Cat# GFP-1010; RRID: AB_2307313 HA.11 Article Title: A ubiquitin variant-based affinity approach selectively identifies substrates of the ubiquitin ligase E6AP in complex with HPV-11 E6 or HPV-16 E6 Article Snippet: Where indicated, HA-tagged proteins were detected by the Article Title: HP1 drives de novo 3D genome reorganization in early Drosophila embryos Article Snippet: HP1 Developmental Studies Hybridoma Bank C1A9 (validated by western blot and ChIP in HP1-KD in ED Fig. 1g and 3b) Article Title: Sumoylation of SAP130 regulates its interaction with FAF1 as well as its protein stability and transcriptional repressor function. Article Snippet: The following antibodies were purchased: Article Title: LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding Article Snippet: Cells were fixed in 10% formaldehyde, permeabilized in 0.1% Triton X-100, and immunostained with antibody against FLAG M2 (Sigma, 1:500), HA.11 (Covance, 1:500), TOM20 (Santa Cruz BioTech, 1:500) or HSP60 (Santa Cruz BioTech, 1:500). Article Title: Tandem Immunoaffinity Purification Using Anti-FLAG and Anti-HA Antibodies. Article Snippet: The immunoaffinity purification of target proteins followed by the identification and characterization of associated proteins by mass spectrometry is a widely used technique.. An immunoaffinity purification bears resemblance to a standard immunoprecipitation; however, the end product for mass spectrometric analysis in the femtomole (10) to attomole (10) range is required to be of exceptional purity.. This high degree of sensitivity in detection renders it of extreme importance to eliminate most if not all of the nonspecific background proteins and can be achieved by performing a tandem affinity purification (TAP). Virus:Article Title: Characterization of the HHV-6B U20 Immunoevasin. Article Snippet: Roseoloviruses (human herpesvirus 6A [HHV-6A], -6B, and -7) infect .90% of the human population during early childhood and are thought to remain latent or persistent throughout the life of the host.. As such, these viruses are among the most pervasive and stealthy of all viruses; they must necessarily excel at escaping immune detection throughout the life of the host, and yet, very little is known about how these viruses so successfully escape host defenses.. Here, we characterize the expression, trafficking, and posttranslational modifications of the HHV6B U20 gene product, which is encoded within a block of genes unique to the roseoloviruses. Article Title: Exploring the Expression and Function of cTyro3, a Candidate Zika Virus Receptor, in the Embryonic Chicken Brain and Inner Ear. Article Snippet: The primary antibodies were diluted in blocking solution as follows: anti-GFP (1:1000, rabbit IgG, Invitrogen, Waltham, MA, USA, A11122), HA.11 (1:500, Article Title: Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca 2+ homeostasis with adipose tissue lipolysis Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alpha-MSH Millipore Cat# AB5087; RRID: AB_91683 Alpha-Tubulin Sigma-Aldrich Cat# T6199; RRID: AB_477583 Alexa Fluor 594 chicken anti-mouse ThermoFisher Cat# A-21201; RRID: AB_2535787 Alexa Fluor 488 chicken anti-rabbit ThermoFisher Cat# A-21441; RRID: AB_2535859 Alexa Fluor 488 donkey anti-sheep ThermoFisher Cat# A-11015; RRID: AB_2534082 Alexa Fluor 594 donkey anti-rabbit ThermoFisher Cat# A-21207; RRID: AB_141637 Alexa Fluor 647 donkey anti-rabbit ThermoFisher Cat# A32795; RRID: AB_2762835 Anti-mouse HRP GE Healthcare UK Limited Cat# NA931; RRID: AB_772210 Anti-Rabbit HRP GE Healthcare UK Limited Cat# NA934; RRID: AB_2722659 b-Actin Sigma-Aldrich Cat# A2066; RRID: AB_476693 GFP Aves Labs Cat# GFP-1010; RRID: AB_2307313 HA.11 Article Title: A ubiquitin variant-based affinity approach selectively identifies substrates of the ubiquitin ligase E6AP in complex with HPV-11 E6 or HPV-16 E6 Article Snippet: Where indicated, HA-tagged proteins were detected by the Article Title: HP1 drives de novo 3D genome reorganization in early Drosophila embryos Article Snippet: HP1 Developmental Studies Hybridoma Bank C1A9 (validated by western blot and ChIP in HP1-KD in ED Fig. 1g and 3b) Article Title: Sumoylation of SAP130 regulates its interaction with FAF1 as well as its protein stability and transcriptional repressor function. Article Snippet: The following antibodies were purchased: Article Title: LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding Article Snippet: Cells were fixed in 10% formaldehyde, permeabilized in 0.1% Triton X-100, and immunostained with antibody against FLAG M2 (Sigma, 1:500), HA.11 (Covance, 1:500), TOM20 (Santa Cruz BioTech, 1:500) or HSP60 (Santa Cruz BioTech, 1:500). Article Title: Tandem Immunoaffinity Purification Using Anti-FLAG and Anti-HA Antibodies. Article Snippet: The immunoaffinity purification of target proteins followed by the identification and characterization of associated proteins by mass spectrometry is a widely used technique.. An immunoaffinity purification bears resemblance to a standard immunoprecipitation; however, the end product for mass spectrometric analysis in the femtomole (10) to attomole (10) range is required to be of exceptional purity.. This high degree of sensitivity in detection renders it of extreme importance to eliminate most if not all of the nonspecific background proteins and can be achieved by performing a tandem affinity purification (TAP). Recombinant:Article Title: Characterization of the HHV-6B U20 Immunoevasin. Article Snippet: Roseoloviruses (human herpesvirus 6A [HHV-6A], -6B, and -7) infect .90% of the human population during early childhood and are thought to remain latent or persistent throughout the life of the host.. As such, these viruses are among the most pervasive and stealthy of all viruses; they must necessarily excel at escaping immune detection throughout the life of the host, and yet, very little is known about how these viruses so successfully escape host defenses.. Here, we characterize the expression, trafficking, and posttranslational modifications of the HHV6B U20 gene product, which is encoded within a block of genes unique to the roseoloviruses. Article Title: Exploring the Expression and Function of cTyro3, a Candidate Zika Virus Receptor, in the Embryonic Chicken Brain and Inner Ear. Article Snippet: The primary antibodies were diluted in blocking solution as follows: anti-GFP (1:1000, rabbit IgG, Invitrogen, Waltham, MA, USA, A11122), HA.11 (1:500, Article Title: Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca 2+ homeostasis with adipose tissue lipolysis Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alpha-MSH Millipore Cat# AB5087; RRID: AB_91683 Alpha-Tubulin Sigma-Aldrich Cat# T6199; RRID: AB_477583 Alexa Fluor 594 chicken anti-mouse ThermoFisher Cat# A-21201; RRID: AB_2535787 Alexa Fluor 488 chicken anti-rabbit ThermoFisher Cat# A-21441; RRID: AB_2535859 Alexa Fluor 488 donkey anti-sheep ThermoFisher Cat# A-11015; RRID: AB_2534082 Alexa Fluor 594 donkey anti-rabbit ThermoFisher Cat# A-21207; RRID: AB_141637 Alexa Fluor 647 donkey anti-rabbit ThermoFisher Cat# A32795; RRID: AB_2762835 Anti-mouse HRP GE Healthcare UK Limited Cat# NA931; RRID: AB_772210 Anti-Rabbit HRP GE Healthcare UK Limited Cat# NA934; RRID: AB_2722659 b-Actin Sigma-Aldrich Cat# A2066; RRID: AB_476693 GFP Aves Labs Cat# GFP-1010; RRID: AB_2307313 HA.11 Article Title: A ubiquitin variant-based affinity approach selectively identifies substrates of the ubiquitin ligase E6AP in complex with HPV-11 E6 or HPV-16 E6 Article Snippet: Where indicated, HA-tagged proteins were detected by the Article Title: HP1 drives de novo 3D genome reorganization in early Drosophila embryos Article Snippet: HP1 Developmental Studies Hybridoma Bank C1A9 (validated by western blot and ChIP in HP1-KD in ED Fig. 1g and 3b) Article Title: Sumoylation of SAP130 regulates its interaction with FAF1 as well as its protein stability and transcriptional repressor function. Article Snippet: The following antibodies were purchased: Article Title: LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding Article Snippet: Cells were fixed in 10% formaldehyde, permeabilized in 0.1% Triton X-100, and immunostained with antibody against FLAG M2 (Sigma, 1:500), HA.11 (Covance, 1:500), TOM20 (Santa Cruz BioTech, 1:500) or HSP60 (Santa Cruz BioTech, 1:500). Article Title: Tandem Immunoaffinity Purification Using Anti-FLAG and Anti-HA Antibodies. Article Snippet: The immunoaffinity purification of target proteins followed by the identification and characterization of associated proteins by mass spectrometry is a widely used technique.. An immunoaffinity purification bears resemblance to a standard immunoprecipitation; however, the end product for mass spectrometric analysis in the femtomole (10) to attomole (10) range is required to be of exceptional purity.. This high degree of sensitivity in detection renders it of extreme importance to eliminate most if not all of the nonspecific background proteins and can be achieved by performing a tandem affinity purification (TAP). Electron Microscopy:Article Title: Characterization of the HHV-6B U20 Immunoevasin. Article Snippet: Roseoloviruses (human herpesvirus 6A [HHV-6A], -6B, and -7) infect .90% of the human population during early childhood and are thought to remain latent or persistent throughout the life of the host.. As such, these viruses are among the most pervasive and stealthy of all viruses; they must necessarily excel at escaping immune detection throughout the life of the host, and yet, very little is known about how these viruses so successfully escape host defenses.. Here, we characterize the expression, trafficking, and posttranslational modifications of the HHV6B U20 gene product, which is encoded within a block of genes unique to the roseoloviruses. Article Title: Exploring the Expression and Function of cTyro3, a Candidate Zika Virus Receptor, in the Embryonic Chicken Brain and Inner Ear. Article Snippet: The primary antibodies were diluted in blocking solution as follows: anti-GFP (1:1000, rabbit IgG, Invitrogen, Waltham, MA, USA, A11122), HA.11 (1:500, Article Title: Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca 2+ homeostasis with adipose tissue lipolysis Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alpha-MSH Millipore Cat# AB5087; RRID: AB_91683 Alpha-Tubulin Sigma-Aldrich Cat# T6199; RRID: AB_477583 Alexa Fluor 594 chicken anti-mouse ThermoFisher Cat# A-21201; RRID: AB_2535787 Alexa Fluor 488 chicken anti-rabbit ThermoFisher Cat# A-21441; RRID: AB_2535859 Alexa Fluor 488 donkey anti-sheep ThermoFisher Cat# A-11015; RRID: AB_2534082 Alexa Fluor 594 donkey anti-rabbit ThermoFisher Cat# A-21207; RRID: AB_141637 Alexa Fluor 647 donkey anti-rabbit ThermoFisher Cat# A32795; RRID: AB_2762835 Anti-mouse HRP GE Healthcare UK Limited Cat# NA931; RRID: AB_772210 Anti-Rabbit HRP GE Healthcare UK Limited Cat# NA934; RRID: AB_2722659 b-Actin Sigma-Aldrich Cat# A2066; RRID: AB_476693 GFP Aves Labs Cat# GFP-1010; RRID: AB_2307313 HA.11 Article Title: A ubiquitin variant-based affinity approach selectively identifies substrates of the ubiquitin ligase E6AP in complex with HPV-11 E6 or HPV-16 E6 Article Snippet: Where indicated, HA-tagged proteins were detected by the Article Title: HP1 drives de novo 3D genome reorganization in early Drosophila embryos Article Snippet: HP1 Developmental Studies Hybridoma Bank C1A9 (validated by western blot and ChIP in HP1-KD in ED Fig. 1g and 3b) Article Title: Sumoylation of SAP130 regulates its interaction with FAF1 as well as its protein stability and transcriptional repressor function. Article Snippet: The following antibodies were purchased: Article Title: LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding Article Snippet: Cells were fixed in 10% formaldehyde, permeabilized in 0.1% Triton X-100, and immunostained with antibody against FLAG M2 (Sigma, 1:500), HA.11 (Covance, 1:500), TOM20 (Santa Cruz BioTech, 1:500) or HSP60 (Santa Cruz BioTech, 1:500). Article Title: Tandem Immunoaffinity Purification Using Anti-FLAG and Anti-HA Antibodies. Article Snippet: The immunoaffinity purification of target proteins followed by the identification and characterization of associated proteins by mass spectrometry is a widely used technique.. An immunoaffinity purification bears resemblance to a standard immunoprecipitation; however, the end product for mass spectrometric analysis in the femtomole (10) to attomole (10) range is required to be of exceptional purity.. This high degree of sensitivity in detection renders it of extreme importance to eliminate most if not all of the nonspecific background proteins and can be achieved by performing a tandem affinity purification (TAP). Western Blot:Article Title: Characterization of the HHV-6B U20 Immunoevasin. Article Snippet: Roseoloviruses (human herpesvirus 6A [HHV-6A], -6B, and -7) infect .90% of the human population during early childhood and are thought to remain latent or persistent throughout the life of the host.. As such, these viruses are among the most pervasive and stealthy of all viruses; they must necessarily excel at escaping immune detection throughout the life of the host, and yet, very little is known about how these viruses so successfully escape host defenses.. Here, we characterize the expression, trafficking, and posttranslational modifications of the HHV6B U20 gene product, which is encoded within a block of genes unique to the roseoloviruses. Article Title: Exploring the Expression and Function of cTyro3, a Candidate Zika Virus Receptor, in the Embryonic Chicken Brain and Inner Ear. Article Snippet: The primary antibodies were diluted in blocking solution as follows: anti-GFP (1:1000, rabbit IgG, Invitrogen, Waltham, MA, USA, A11122), HA.11 (1:500, Article Title: Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca 2+ homeostasis with adipose tissue lipolysis Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alpha-MSH Millipore Cat# AB5087; RRID: AB_91683 Alpha-Tubulin Sigma-Aldrich Cat# T6199; RRID: AB_477583 Alexa Fluor 594 chicken anti-mouse ThermoFisher Cat# A-21201; RRID: AB_2535787 Alexa Fluor 488 chicken anti-rabbit ThermoFisher Cat# A-21441; RRID: AB_2535859 Alexa Fluor 488 donkey anti-sheep ThermoFisher Cat# A-11015; RRID: AB_2534082 Alexa Fluor 594 donkey anti-rabbit ThermoFisher Cat# A-21207; RRID: AB_141637 Alexa Fluor 647 donkey anti-rabbit ThermoFisher Cat# A32795; RRID: AB_2762835 Anti-mouse HRP GE Healthcare UK Limited Cat# NA931; RRID: AB_772210 Anti-Rabbit HRP GE Healthcare UK Limited Cat# NA934; RRID: AB_2722659 b-Actin Sigma-Aldrich Cat# A2066; RRID: AB_476693 GFP Aves Labs Cat# GFP-1010; RRID: AB_2307313 HA.11 Article Title: A ubiquitin variant-based affinity approach selectively identifies substrates of the ubiquitin ligase E6AP in complex with HPV-11 E6 or HPV-16 E6 Article Snippet: Where indicated, HA-tagged proteins were detected by the Article Title: HP1 drives de novo 3D genome reorganization in early Drosophila embryos Article Snippet: HP1 Developmental Studies Hybridoma Bank C1A9 (validated by western blot and ChIP in HP1-KD in ED Fig. 1g and 3b) Article Title: Sumoylation of SAP130 regulates its interaction with FAF1 as well as its protein stability and transcriptional repressor function. Article Snippet: The following antibodies were purchased: Article Title: LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding Article Snippet: Cells were fixed in 10% formaldehyde, permeabilized in 0.1% Triton X-100, and immunostained with antibody against FLAG M2 (Sigma, 1:500), HA.11 (Covance, 1:500), TOM20 (Santa Cruz BioTech, 1:500) or HSP60 (Santa Cruz BioTech, 1:500). Article Title: Tandem Immunoaffinity Purification Using Anti-FLAG and Anti-HA Antibodies. Article Snippet: The immunoaffinity purification of target proteins followed by the identification and characterization of associated proteins by mass spectrometry is a widely used technique.. An immunoaffinity purification bears resemblance to a standard immunoprecipitation; however, the end product for mass spectrometric analysis in the femtomole (10) to attomole (10) range is required to be of exceptional purity.. This high degree of sensitivity in detection renders it of extreme importance to eliminate most if not all of the nonspecific background proteins and can be achieved by performing a tandem affinity purification (TAP). Chromatin Immunoprecipitation:Article Title: Characterization of the HHV-6B U20 Immunoevasin. Article Snippet: Roseoloviruses (human herpesvirus 6A [HHV-6A], -6B, and -7) infect .90% of the human population during early childhood and are thought to remain latent or persistent throughout the life of the host.. As such, these viruses are among the most pervasive and stealthy of all viruses; they must necessarily excel at escaping immune detection throughout the life of the host, and yet, very little is known about how these viruses so successfully escape host defenses.. Here, we characterize the expression, trafficking, and posttranslational modifications of the HHV6B U20 gene product, which is encoded within a block of genes unique to the roseoloviruses. Article Title: Exploring the Expression and Function of cTyro3, a Candidate Zika Virus Receptor, in the Embryonic Chicken Brain and Inner Ear. Article Snippet: The primary antibodies were diluted in blocking solution as follows: anti-GFP (1:1000, rabbit IgG, Invitrogen, Waltham, MA, USA, A11122), HA.11 (1:500, Article Title: Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca 2+ homeostasis with adipose tissue lipolysis Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alpha-MSH Millipore Cat# AB5087; RRID: AB_91683 Alpha-Tubulin Sigma-Aldrich Cat# T6199; RRID: AB_477583 Alexa Fluor 594 chicken anti-mouse ThermoFisher Cat# A-21201; RRID: AB_2535787 Alexa Fluor 488 chicken anti-rabbit ThermoFisher Cat# A-21441; RRID: AB_2535859 Alexa Fluor 488 donkey anti-sheep ThermoFisher Cat# A-11015; RRID: AB_2534082 Alexa Fluor 594 donkey anti-rabbit ThermoFisher Cat# A-21207; RRID: AB_141637 Alexa Fluor 647 donkey anti-rabbit ThermoFisher Cat# A32795; RRID: AB_2762835 Anti-mouse HRP GE Healthcare UK Limited Cat# NA931; RRID: AB_772210 Anti-Rabbit HRP GE Healthcare UK Limited Cat# NA934; RRID: AB_2722659 b-Actin Sigma-Aldrich Cat# A2066; RRID: AB_476693 GFP Aves Labs Cat# GFP-1010; RRID: AB_2307313 HA.11 Article Title: A ubiquitin variant-based affinity approach selectively identifies substrates of the ubiquitin ligase E6AP in complex with HPV-11 E6 or HPV-16 E6 Article Snippet: Where indicated, HA-tagged proteins were detected by the Article Title: HP1 drives de novo 3D genome reorganization in early Drosophila embryos Article Snippet: HP1 Developmental Studies Hybridoma Bank C1A9 (validated by western blot and ChIP in HP1-KD in ED Fig. 1g and 3b) Article Title: Sumoylation of SAP130 regulates its interaction with FAF1 as well as its protein stability and transcriptional repressor function. Article Snippet: The following antibodies were purchased: Article Title: LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding Article Snippet: Cells were fixed in 10% formaldehyde, permeabilized in 0.1% Triton X-100, and immunostained with antibody against FLAG M2 (Sigma, 1:500), HA.11 (Covance, 1:500), TOM20 (Santa Cruz BioTech, 1:500) or HSP60 (Santa Cruz BioTech, 1:500). Article Title: Tandem Immunoaffinity Purification Using Anti-FLAG and Anti-HA Antibodies. Article Snippet: The immunoaffinity purification of target proteins followed by the identification and characterization of associated proteins by mass spectrometry is a widely used technique.. An immunoaffinity purification bears resemblance to a standard immunoprecipitation; however, the end product for mass spectrometric analysis in the femtomole (10) to attomole (10) range is required to be of exceptional purity.. This high degree of sensitivity in detection renders it of extreme importance to eliminate most if not all of the nonspecific background proteins and can be achieved by performing a tandem affinity purification (TAP). Next-Generation Sequencing:Article Title: Characterization of the HHV-6B U20 Immunoevasin. Article Snippet: Roseoloviruses (human herpesvirus 6A [HHV-6A], -6B, and -7) infect .90% of the human population during early childhood and are thought to remain latent or persistent throughout the life of the host.. As such, these viruses are among the most pervasive and stealthy of all viruses; they must necessarily excel at escaping immune detection throughout the life of the host, and yet, very little is known about how these viruses so successfully escape host defenses.. Here, we characterize the expression, trafficking, and posttranslational modifications of the HHV6B U20 gene product, which is encoded within a block of genes unique to the roseoloviruses. Article Title: Exploring the Expression and Function of cTyro3, a Candidate Zika Virus Receptor, in the Embryonic Chicken Brain and Inner Ear. Article Snippet: The primary antibodies were diluted in blocking solution as follows: anti-GFP (1:1000, rabbit IgG, Invitrogen, Waltham, MA, USA, A11122), HA.11 (1:500, Article Title: Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca 2+ homeostasis with adipose tissue lipolysis Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alpha-MSH Millipore Cat# AB5087; RRID: AB_91683 Alpha-Tubulin Sigma-Aldrich Cat# T6199; RRID: AB_477583 Alexa Fluor 594 chicken anti-mouse ThermoFisher Cat# A-21201; RRID: AB_2535787 Alexa Fluor 488 chicken anti-rabbit ThermoFisher Cat# A-21441; RRID: AB_2535859 Alexa Fluor 488 donkey anti-sheep ThermoFisher Cat# A-11015; RRID: AB_2534082 Alexa Fluor 594 donkey anti-rabbit ThermoFisher Cat# A-21207; RRID: AB_141637 Alexa Fluor 647 donkey anti-rabbit ThermoFisher Cat# A32795; RRID: AB_2762835 Anti-mouse HRP GE Healthcare UK Limited Cat# NA931; RRID: AB_772210 Anti-Rabbit HRP GE Healthcare UK Limited Cat# NA934; RRID: AB_2722659 b-Actin Sigma-Aldrich Cat# A2066; RRID: AB_476693 GFP Aves Labs Cat# GFP-1010; RRID: AB_2307313 HA.11 Article Title: A ubiquitin variant-based affinity approach selectively identifies substrates of the ubiquitin ligase E6AP in complex with HPV-11 E6 or HPV-16 E6 Article Snippet: Where indicated, HA-tagged proteins were detected by the Article Title: HP1 drives de novo 3D genome reorganization in early Drosophila embryos Article Snippet: HP1 Developmental Studies Hybridoma Bank C1A9 (validated by western blot and ChIP in HP1-KD in ED Fig. 1g and 3b) Article Title: Sumoylation of SAP130 regulates its interaction with FAF1 as well as its protein stability and transcriptional repressor function. Article Snippet: The following antibodies were purchased: Article Title: LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding Article Snippet: Cells were fixed in 10% formaldehyde, permeabilized in 0.1% Triton X-100, and immunostained with antibody against FLAG M2 (Sigma, 1:500), HA.11 (Covance, 1:500), TOM20 (Santa Cruz BioTech, 1:500) or HSP60 (Santa Cruz BioTech, 1:500). Article Title: Tandem Immunoaffinity Purification Using Anti-FLAG and Anti-HA Antibodies. Article Snippet: The immunoaffinity purification of target proteins followed by the identification and characterization of associated proteins by mass spectrometry is a widely used technique.. An immunoaffinity purification bears resemblance to a standard immunoprecipitation; however, the end product for mass spectrometric analysis in the femtomole (10) to attomole (10) range is required to be of exceptional purity.. This high degree of sensitivity in detection renders it of extreme importance to eliminate most if not all of the nonspecific background proteins and can be achieved by performing a tandem affinity purification (TAP). Immunofluorescence:Article Title: Characterization of the HHV-6B U20 Immunoevasin. Article Snippet: Roseoloviruses (human herpesvirus 6A [HHV-6A], -6B, and -7) infect .90% of the human population during early childhood and are thought to remain latent or persistent throughout the life of the host.. As such, these viruses are among the most pervasive and stealthy of all viruses; they must necessarily excel at escaping immune detection throughout the life of the host, and yet, very little is known about how these viruses so successfully escape host defenses.. Here, we characterize the expression, trafficking, and posttranslational modifications of the HHV6B U20 gene product, which is encoded within a block of genes unique to the roseoloviruses. Article Title: Exploring the Expression and Function of cTyro3, a Candidate Zika Virus Receptor, in the Embryonic Chicken Brain and Inner Ear. Article Snippet: The primary antibodies were diluted in blocking solution as follows: anti-GFP (1:1000, rabbit IgG, Invitrogen, Waltham, MA, USA, A11122), HA.11 (1:500, Article Title: Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca 2+ homeostasis with adipose tissue lipolysis Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alpha-MSH Millipore Cat# AB5087; RRID: AB_91683 Alpha-Tubulin Sigma-Aldrich Cat# T6199; RRID: AB_477583 Alexa Fluor 594 chicken anti-mouse ThermoFisher Cat# A-21201; RRID: AB_2535787 Alexa Fluor 488 chicken anti-rabbit ThermoFisher Cat# A-21441; RRID: AB_2535859 Alexa Fluor 488 donkey anti-sheep ThermoFisher Cat# A-11015; RRID: AB_2534082 Alexa Fluor 594 donkey anti-rabbit ThermoFisher Cat# A-21207; RRID: AB_141637 Alexa Fluor 647 donkey anti-rabbit ThermoFisher Cat# A32795; RRID: AB_2762835 Anti-mouse HRP GE Healthcare UK Limited Cat# NA931; RRID: AB_772210 Anti-Rabbit HRP GE Healthcare UK Limited Cat# NA934; RRID: AB_2722659 b-Actin Sigma-Aldrich Cat# A2066; RRID: AB_476693 GFP Aves Labs Cat# GFP-1010; RRID: AB_2307313 HA.11 Article Title: A ubiquitin variant-based affinity approach selectively identifies substrates of the ubiquitin ligase E6AP in complex with HPV-11 E6 or HPV-16 E6 Article Snippet: Where indicated, HA-tagged proteins were detected by the Article Title: HP1 drives de novo 3D genome reorganization in early Drosophila embryos Article Snippet: HP1 Developmental Studies Hybridoma Bank C1A9 (validated by western blot and ChIP in HP1-KD in ED Fig. 1g and 3b) Article Title: Sumoylation of SAP130 regulates its interaction with FAF1 as well as its protein stability and transcriptional repressor function. Article Snippet: The following antibodies were purchased: Article Title: LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding Article Snippet: Cells were fixed in 10% formaldehyde, permeabilized in 0.1% Triton X-100, and immunostained with antibody against FLAG M2 (Sigma, 1:500), HA.11 (Covance, 1:500), TOM20 (Santa Cruz BioTech, 1:500) or HSP60 (Santa Cruz BioTech, 1:500). Article Title: Tandem Immunoaffinity Purification Using Anti-FLAG and Anti-HA Antibodies. Article Snippet: The immunoaffinity purification of target proteins followed by the identification and characterization of associated proteins by mass spectrometry is a widely used technique.. An immunoaffinity purification bears resemblance to a standard immunoprecipitation; however, the end product for mass spectrometric analysis in the femtomole (10) to attomole (10) range is required to be of exceptional purity.. This high degree of sensitivity in detection renders it of extreme importance to eliminate most if not all of the nonspecific background proteins and can be achieved by performing a tandem affinity purification (TAP). Biomarker Discovery:Article Title: Characterization of the HHV-6B U20 Immunoevasin. Article Snippet: Roseoloviruses (human herpesvirus 6A [HHV-6A], -6B, and -7) infect .90% of the human population during early childhood and are thought to remain latent or persistent throughout the life of the host.. As such, these viruses are among the most pervasive and stealthy of all viruses; they must necessarily excel at escaping immune detection throughout the life of the host, and yet, very little is known about how these viruses so successfully escape host defenses.. Here, we characterize the expression, trafficking, and posttranslational modifications of the HHV6B U20 gene product, which is encoded within a block of genes unique to the roseoloviruses. Article Title: Exploring the Expression and Function of cTyro3, a Candidate Zika Virus Receptor, in the Embryonic Chicken Brain and Inner Ear. Article Snippet: The primary antibodies were diluted in blocking solution as follows: anti-GFP (1:1000, rabbit IgG, Invitrogen, Waltham, MA, USA, A11122), HA.11 (1:500, Article Title: Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca 2+ homeostasis with adipose tissue lipolysis Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alpha-MSH Millipore Cat# AB5087; RRID: AB_91683 Alpha-Tubulin Sigma-Aldrich Cat# T6199; RRID: AB_477583 Alexa Fluor 594 chicken anti-mouse ThermoFisher Cat# A-21201; RRID: AB_2535787 Alexa Fluor 488 chicken anti-rabbit ThermoFisher Cat# A-21441; RRID: AB_2535859 Alexa Fluor 488 donkey anti-sheep ThermoFisher Cat# A-11015; RRID: AB_2534082 Alexa Fluor 594 donkey anti-rabbit ThermoFisher Cat# A-21207; RRID: AB_141637 Alexa Fluor 647 donkey anti-rabbit ThermoFisher Cat# A32795; RRID: AB_2762835 Anti-mouse HRP GE Healthcare UK Limited Cat# NA931; RRID: AB_772210 Anti-Rabbit HRP GE Healthcare UK Limited Cat# NA934; RRID: AB_2722659 b-Actin Sigma-Aldrich Cat# A2066; RRID: AB_476693 GFP Aves Labs Cat# GFP-1010; RRID: AB_2307313 HA.11 Article Title: A ubiquitin variant-based affinity approach selectively identifies substrates of the ubiquitin ligase E6AP in complex with HPV-11 E6 or HPV-16 E6 Article Snippet: Where indicated, HA-tagged proteins were detected by the Article Title: HP1 drives de novo 3D genome reorganization in early Drosophila embryos Article Snippet: HP1 Developmental Studies Hybridoma Bank C1A9 (validated by western blot and ChIP in HP1-KD in ED Fig. 1g and 3b) Article Title: Sumoylation of SAP130 regulates its interaction with FAF1 as well as its protein stability and transcriptional repressor function. Article Snippet: The following antibodies were purchased: Article Title: LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding Article Snippet: Cells were fixed in 10% formaldehyde, permeabilized in 0.1% Triton X-100, and immunostained with antibody against FLAG M2 (Sigma, 1:500), HA.11 (Covance, 1:500), TOM20 (Santa Cruz BioTech, 1:500) or HSP60 (Santa Cruz BioTech, 1:500). Article Title: Tandem Immunoaffinity Purification Using Anti-FLAG and Anti-HA Antibodies. Article Snippet: The immunoaffinity purification of target proteins followed by the identification and characterization of associated proteins by mass spectrometry is a widely used technique.. An immunoaffinity purification bears resemblance to a standard immunoprecipitation; however, the end product for mass spectrometric analysis in the femtomole (10) to attomole (10) range is required to be of exceptional purity.. This high degree of sensitivity in detection renders it of extreme importance to eliminate most if not all of the nonspecific background proteins and can be achieved by performing a tandem affinity purification (TAP). Clone Assay:Article Title: Characterization of the HHV-6B U20 Immunoevasin. Article Snippet: Roseoloviruses (human herpesvirus 6A [HHV-6A], -6B, and -7) infect .90% of the human population during early childhood and are thought to remain latent or persistent throughout the life of the host.. As such, these viruses are among the most pervasive and stealthy of all viruses; they must necessarily excel at escaping immune detection throughout the life of the host, and yet, very little is known about how these viruses so successfully escape host defenses.. Here, we characterize the expression, trafficking, and posttranslational modifications of the HHV6B U20 gene product, which is encoded within a block of genes unique to the roseoloviruses. Article Title: Exploring the Expression and Function of cTyro3, a Candidate Zika Virus Receptor, in the Embryonic Chicken Brain and Inner Ear. Article Snippet: The primary antibodies were diluted in blocking solution as follows: anti-GFP (1:1000, rabbit IgG, Invitrogen, Waltham, MA, USA, A11122), HA.11 (1:500, Article Title: Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca 2+ homeostasis with adipose tissue lipolysis Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alpha-MSH Millipore Cat# AB5087; RRID: AB_91683 Alpha-Tubulin Sigma-Aldrich Cat# T6199; RRID: AB_477583 Alexa Fluor 594 chicken anti-mouse ThermoFisher Cat# A-21201; RRID: AB_2535787 Alexa Fluor 488 chicken anti-rabbit ThermoFisher Cat# A-21441; RRID: AB_2535859 Alexa Fluor 488 donkey anti-sheep ThermoFisher Cat# A-11015; RRID: AB_2534082 Alexa Fluor 594 donkey anti-rabbit ThermoFisher Cat# A-21207; RRID: AB_141637 Alexa Fluor 647 donkey anti-rabbit ThermoFisher Cat# A32795; RRID: AB_2762835 Anti-mouse HRP GE Healthcare UK Limited Cat# NA931; RRID: AB_772210 Anti-Rabbit HRP GE Healthcare UK Limited Cat# NA934; RRID: AB_2722659 b-Actin Sigma-Aldrich Cat# A2066; RRID: AB_476693 GFP Aves Labs Cat# GFP-1010; RRID: AB_2307313 HA.11 Article Title: A ubiquitin variant-based affinity approach selectively identifies substrates of the ubiquitin ligase E6AP in complex with HPV-11 E6 or HPV-16 E6 Article Snippet: Where indicated, HA-tagged proteins were detected by the Article Title: HP1 drives de novo 3D genome reorganization in early Drosophila embryos Article Snippet: HP1 Developmental Studies Hybridoma Bank C1A9 (validated by western blot and ChIP in HP1-KD in ED Fig. 1g and 3b) Article Title: Sumoylation of SAP130 regulates its interaction with FAF1 as well as its protein stability and transcriptional repressor function. Article Snippet: The following antibodies were purchased: Article Title: LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding Article Snippet: Cells were fixed in 10% formaldehyde, permeabilized in 0.1% Triton X-100, and immunostained with antibody against FLAG M2 (Sigma, 1:500), HA.11 (Covance, 1:500), TOM20 (Santa Cruz BioTech, 1:500) or HSP60 (Santa Cruz BioTech, 1:500). Article Title: Tandem Immunoaffinity Purification Using Anti-FLAG and Anti-HA Antibodies. Article Snippet: The immunoaffinity purification of target proteins followed by the identification and characterization of associated proteins by mass spectrometry is a widely used technique.. An immunoaffinity purification bears resemblance to a standard immunoprecipitation; however, the end product for mass spectrometric analysis in the femtomole (10) to attomole (10) range is required to be of exceptional purity.. This high degree of sensitivity in detection renders it of extreme importance to eliminate most if not all of the nonspecific background proteins and can be achieved by performing a tandem affinity purification (TAP). Binding Assay:Article Title: Characterization of the HHV-6B U20 Immunoevasin. Article Snippet: Roseoloviruses (human herpesvirus 6A [HHV-6A], -6B, and -7) infect .90% of the human population during early childhood and are thought to remain latent or persistent throughout the life of the host.. As such, these viruses are among the most pervasive and stealthy of all viruses; they must necessarily excel at escaping immune detection throughout the life of the host, and yet, very little is known about how these viruses so successfully escape host defenses.. Here, we characterize the expression, trafficking, and posttranslational modifications of the HHV6B U20 gene product, which is encoded within a block of genes unique to the roseoloviruses. Article Title: Exploring the Expression and Function of cTyro3, a Candidate Zika Virus Receptor, in the Embryonic Chicken Brain and Inner Ear. Article Snippet: The primary antibodies were diluted in blocking solution as follows: anti-GFP (1:1000, rabbit IgG, Invitrogen, Waltham, MA, USA, A11122), HA.11 (1:500, Article Title: Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca 2+ homeostasis with adipose tissue lipolysis Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alpha-MSH Millipore Cat# AB5087; RRID: AB_91683 Alpha-Tubulin Sigma-Aldrich Cat# T6199; RRID: AB_477583 Alexa Fluor 594 chicken anti-mouse ThermoFisher Cat# A-21201; RRID: AB_2535787 Alexa Fluor 488 chicken anti-rabbit ThermoFisher Cat# A-21441; RRID: AB_2535859 Alexa Fluor 488 donkey anti-sheep ThermoFisher Cat# A-11015; RRID: AB_2534082 Alexa Fluor 594 donkey anti-rabbit ThermoFisher Cat# A-21207; RRID: AB_141637 Alexa Fluor 647 donkey anti-rabbit ThermoFisher Cat# A32795; RRID: AB_2762835 Anti-mouse HRP GE Healthcare UK Limited Cat# NA931; RRID: AB_772210 Anti-Rabbit HRP GE Healthcare UK Limited Cat# NA934; RRID: AB_2722659 b-Actin Sigma-Aldrich Cat# A2066; RRID: AB_476693 GFP Aves Labs Cat# GFP-1010; RRID: AB_2307313 HA.11 Article Title: A ubiquitin variant-based affinity approach selectively identifies substrates of the ubiquitin ligase E6AP in complex with HPV-11 E6 or HPV-16 E6 Article Snippet: Where indicated, HA-tagged proteins were detected by the Article Title: HP1 drives de novo 3D genome reorganization in early Drosophila embryos Article Snippet: HP1 Developmental Studies Hybridoma Bank C1A9 (validated by western blot and ChIP in HP1-KD in ED Fig. 1g and 3b) Article Title: Sumoylation of SAP130 regulates its interaction with FAF1 as well as its protein stability and transcriptional repressor function. Article Snippet: The following antibodies were purchased: Article Title: LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding Article Snippet: Cells were fixed in 10% formaldehyde, permeabilized in 0.1% Triton X-100, and immunostained with antibody against FLAG M2 (Sigma, 1:500), HA.11 (Covance, 1:500), TOM20 (Santa Cruz BioTech, 1:500) or HSP60 (Santa Cruz BioTech, 1:500). Article Title: Tandem Immunoaffinity Purification Using Anti-FLAG and Anti-HA Antibodies. Article Snippet: The immunoaffinity purification of target proteins followed by the identification and characterization of associated proteins by mass spectrometry is a widely used technique.. An immunoaffinity purification bears resemblance to a standard immunoprecipitation; however, the end product for mass spectrometric analysis in the femtomole (10) to attomole (10) range is required to be of exceptional purity.. This high degree of sensitivity in detection renders it of extreme importance to eliminate most if not all of the nonspecific background proteins and can be achieved by performing a tandem affinity purification (TAP). |